GATCLIQUID ONCOTARGET

SKU#B10161200

Confident monitoring of selected tumour-related mutations

GATCLIQUID ONCOTARGET is a service for highly sensitive monitoring of the most important genomic alteration in a particular cancer case. Based on droplet digital PCR (ddPCR) quantification, the product enables evaluation of single mutations on circulating tumour DNA (ctDNA) with unprecedented analytical sensitivity, uniformity and reproducibility. Due to its noninvasive nature, the product can be used to serially monitor any relevant cancer biomarker from liquid biopsy samples to deliver rapid, clear-cut answers to researchers, clinicians and their patients.

Highlights of GATCLIQUID ONCOTARGET

  • Low-level tumour allele detection in blood with ddPCR technology
  • Detection limit of individual mutations in ctDNA with an allele frequency of as low as 0.1% 
  • Improved precision, uniformity and reproducibility
  • Cost-effective disease surveillance

Service required

Please select at least one of these options.

Pre-Sequencing required

Isolation of cell-free DNA

GATCLIQUID ONCOTARGET

GATCLIQUID ONCOTARGET

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ISO9001 Certificate - GATC BiotechISO17025 Accreditation - GATC Biotech

Product Details

Product details

Full-service package offered by ONCOTARGET includes the following:

  • Droplet digital PCR (ddPCR) analysis of circulating tumour DNA (ctDNA) with millions of picoliter-sized droplets
  • Low-input DNA processing from as little as 10 ng DNA as starting material
  • Increased signal-to-noise ratio 
  • Analytical sensitivity down to 0.1% 
  • Detection of rare targets with ±10% precision in quantification
  • Absolute answers indicating either presence or absence of mutation
  • Minimal contamination from a closed system
  • Cost-efficient evaluation of single mutations

Accepted starting material for ONCOTARGET

  • 4 mL plasma sample

Detection technology used for ONCOTARGET

  • Droplet digital PCR

Deliverables of ONCOTARGET

  • Detection levels of a single tumour-specific mutation

The product is available for research use only (RUO).

Additional Information

Our integrated Diagnostic Solution for tumour tracking takes advantage of noninvasive liquid biopsy samples that are subjected to leading ddPCR technologies. The approach offers the highest sensitivity in quantifying rare and unique tumour-associated mutations that are most relevant to the cancer of each individual patient.

The simplicity and cost-efficiency of the service support repeat analysis of ctDNA for rapid and conclusive detection of a selected cancer biomarker throughout the treatment process.

ONCOTARGET can be used to facilitate personalised medicine by keeping better track of cancer progression on an individual patient basis.

ONCOTARGET - Workflow
Figure 1. Workflow GATCLIQUID ONCOTARGET

Typical applications for GATCLIQUID ONCOTARGET include the following:

  • Quantification of mutation levels over time
  • Detection of very rare tumour-associated mutations 
  • Studies on tumour evolution

ONCOTARGET - List of genes

Table 1. List of genes available for GATCLIQUID ONCOTARGET

GATCLIQUID ONCOTARGET can be used for clinical applications such as:

  • Evaluation of tumour burden
  • Controlling for minimal residual disease
  • Serial tumour monitoring of low-level mutations after treatment
  • Pharmacodynamics of targeted therapy
  • Early detection of relapse

ONCOTARGET - mutations related to cancer

Table 2. List of tumour-associated mutations in ONCOTARGET

The product is available for research use only (RUO).

FAQs

1. What kind of samples can be processed with ONCOTARGET?
For ONCOTARGET, we accept fresh or retained plasma samples. Please refer to our GATCLIQUID sample preparation sheet (see "Files" section). The ctDNA is extracted from the blood plasma fraction.
For starting material such as DNA isolated from FFPE and tissue samples, please refer to our product for solid-tumour profiling (coming soon).

2. How much starting material is required?
In general we require 4 mL of plasma for ctDNA testing.

3. How does ddPCR work?
This PCR approach is based on the partitioning of a PCR mixture into millions of water/oil-emulsion droplets, each of which acts as an individual reaction. Every single droplet can be defined as either positive or negative, based on its fluorescent amplitude. By quantifying the proportion of positive droplets, the total number of target templates can be calculated and absolute DNA levels can be precisely determined without the need for a standard curve.

4. What kind of quality controls do you perform?
The quality and quantity of each sample will be determined upon receiving the sample. Further quality controls are performed at various steps of the process.

5. How should I choose between ONCOEXOME, ONCOPANEL and ONCOTARGET?
All three tests are based on liquid biopsy. The three products serve as powerful non-invasive tools for real-time cancer profiling with the ability to capture the entire heterogeneity of the disease.

GATCLIQUID ONCOEXOME is the first commercially available service for whole exome sequencing (WES) of ctDNA. With this service, you can obtain a comprehensive profile of all mutations present in the protein coding regions of the genome. This product is suitable for a preliminary look at the genome of a suspected cancer patient, in cases where limited information regarding clinically relevant mutations is available.

GATCLIQUID ONCOPANEL is a comprehensive NGS panel for profiling important cancer mutations. It uses single- molecule PCR to target 50 known cancer genes including tumour suppressors, mutation hotspots and drug- resistance markers. The panel sets a new industry standard in terms of sequence coverage and uniformity with a sensitivity of about 1%.

GATCLIQUID ONCOTARGET facilitates ultra-sensitive detection of clinically relevant cancer-specific point mutations, insertions, deletions and gene fusions in circulating tumour DNA down to an allele frequency of 0.1%. This approach is suitable for treatment response monitoring as it is sensitive enough to detect a relapse at a very early stage.

6. How do I obtain my results and what form will they be in when delivered?
All deliverables can be downloaded via your secure myGATC online account.

7. How soon after the blood draw should I ship the sample?
Blood samples have to be shipped to GATC Biotech within 24 hours after drawing blood. The sample must be stored in BCT Streck tubes. Samples may be stored and delivered at room temperature. Plasma samples have to be shipped on dry ice.

8. How many samples can I submit?
GATC Biotech accepts any number of samples in multiples of seven.

9. Where should I send my samples?
Please mail your samples to:
GATC Biotech AG
European Genome and Diagnostics Centre
Jakob-Stadler-Platz 7
78467 Konstanz
Germany

Please do not use GATC Collection Points for shipping NextGen samples, because this will delay sample arrival at the appropriate destination.

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