Overview – What is gene panel sequencing?
Targeted gene panels apply next-generation sequencing (NGS) technology to interrogate the mutation status of multiple genomic regions of interest simultaneously. The focused panels include specific regions of the genome that are associated with a disease or phenotype of interest. Gene panels can help researchers discover point mutations, insertions/deletions, copy number variations and translocations that could be missed with traditional sequencing techniques.
The panel can be either premade or custom designed. The most common predesigned panels contain genes of clinical relevance linked to cancer, Mendelian diseases, cardiac disorders and neurodegenerative conditions. DNA from a variety of sample materials, including FFPE, can be targeted with this approach.
By focusing only on key regions of interest, targeted gene panels help reduce sequencing time and simplify data analysis.
Applications – What are the advantages of targeted gene panels?
- Cost-effective findings for clinical studies on Mendelian diseases and cancer
- Deep coverage of genes and genomic regions of interest
- Identification of rare variants at low allele frequencies
Workflow – Gene panel sequencing methods & technologies
Different methods for the establishment of gene panels are available. Often, target enrichment occurs via solution hybridisation, where probes pull down the genomic regions that they are specific to. Alternatively, enrichment via PCR can be applied, where each targeted region is amplified via a distinct pair of primers in a multiplexed reaction. Other methods for target enrichment include hybridisation microarrays and alternative forms of PCR multiplexing.
Depending on the method used and the project requirements, thousands of DNA targets can be sequenced simultaneously with next-generation sequencing technologies.
Scientific expertise: gene-panel sequencing
GATC Biotech is an expert at targeted sequencing, including sequencing of multigene panels. The company has years of experience, particularly with cancer panels. GATC Biotech has designed and sequenced cancer panels with hundreds of known cancer genes that contain clinically relevant mutations. The company can also offer expertise in the establishment of custom gene panels for novel organisms or for targeting cellular processes outside of the scope of predesigned panels.
Find here, a list of selected research articles supported by GATC Biotech’s sequencing products, as well as the latest publications on targeted sequencing.
Products related to gene panel sequencing
Would you like to interrogate all known mutations associated with a certain disease or a phenotype simultaneously? Then try targeted gene-panel sequencing (INVIEW ONCOPANEL, GATCLIQUID ONCOPANEL) from GATC Biotech. From predesigned to custom panels, see how our expertise can help advance your project.
Are you sceptical that one gene panel can capture all genes of interest and relevant mutations? Then whole-exome sequencing might be the method for you. Try our INVIEW HUMAN EXOME product and explore genetic variation across all protein-coding regions of the genome. A wide variety of starting materials can be used, including FFPE samples. Our GATCLIQUID ONCOTARGET product for ultra-sensitive detection of genomic aberrations uses droplet digital PCR (ddPCR) and can be used for targeting a single or a few important mutations in a particular sample.
Alternatively, the entire genome of an organism of interest can be efficiently captured with our established whole-genome sequencing (WGS) service.
For other cases where only a few targeted genes from a small number of samples need to be enriched, see how our Sanger sequencing services can be used to your advantage.
Further reading on targeted sequencing of gene panels
Saudi Mendeliome, G. Comprehensive gene panels provide advantages over clinical exome sequencing for Mendelian diseases. Genome Biol. 16, 134 (2015).
Lohmann, K., Klein, C. Next generation sequencing and the future of genetic diagnosis. Neurotherapeutics. 4, 699 – 707 (2014).