Overview - What is chromatin immunoprecipitation sequencing (ChIP-seq)?
One of the most popular techniques for exploring DNA-protein interaction is ChIP-Seq or ChIP sequencing. ChIP-Seq combines chromatin immunoprecipitation (ChIP) with next-generation sequencing (NGS) to identify the genomic loci of DNA-protein binding sites. ChIP-Seq can be used to investigate any protein of interest and locate potential binding sites in the entire genome of a specific organism. The method is applicable to research on histone modifications, DNA methylation, nucleosome positioning and functional elements of the genome.
ChIP-Seq experiments have helped improve our understanding of normal and abnormal transcription and gene regulation, cell cycle checkpoint control, DNA synthesis, DNA repair, immune response, signal transduction, embryonic development, viral protein-human genome associations and much more.
Applications - What are the advantages of ChIP-Seq?
The immunoprecipitation NGS-based technique is ideal for exploring DNA and histone modifications, as well as DNA-transcription factor links, because the meth
- Generates high resolution data with a low signal-to-noise ratio
- Functions for samples with low-input DNA
- Interrogates DNA targets for protein-binding sites or is applied for chromatin modifications in the entire genome
- Suited for nearly any organism
- Can be used to explore any chromatin-associated protein of interest
Workflow - Methods & technologies for ChIP-Seq
Chromatin is a DNA packaging complex mainly composed of nucleic acids and histones. During chromatin immunoprecipitation (ChIP), DNA-binding proteins are crosslinked to DNA found in chromatin complexes in vivo. The cross-linked DNA fragments are sheared, and protein-bound DNA fragments are selectively immunoprecipitated using antibodies specific to the protein of interest. The immunoprecipitated DNA fragments are most often analysed with microarrays (ChIP-Chip) or NGS (ChIP-Seq).
ChIP-Chip vs. ChIP-Seq
ChIP-Chip uses micorarrays to analyse DNA-protein binding sites. The technique can provide a global view of DNA-protein interactions in a relatively inexpensive manner. ChIP-Seq is increasingly displacing ChIP-Chip, as the next-generation sequencing-based method offers several advantages over ChIP-Chip. ChIP-Seq is applicable to nearly any organism and requires less input DNA for more sensitive and more specific protein-binding-site identification compared to ChIP-Chip. Due to its higher sensitivity, ChIP-Seq is also more efficient at discovering unknown protein binding sites.
Scientific expertise: ChIP-Seq
GATC Biotech has processed numerous ChiP-Seq samples with leading sequencing technologies and competent bioinformatics analysis to deliver high-quality data to satisfied customers. With GATC Biotech’s optimised workflows, the amount of bioinformatics data analysis following sequencing is substantially reduced without affecting data quality.
Find here, a list of selected research articles supported by GATC Biotech’s sequencing products, including articles on epigenetics.
Related products to ChIP-Seq
Convinced ChIP-Seq is the experiment that will move your research ahead? Then take advantage of our ChIP-Seq service. Simply send us your ChIP-enriched DNA and we will do the rest.
Contact us for more information.
Further reading on ChIP-Seq
Mundade, R., Ozer, H.G., Wie, H., Prabhu, L., Lu, T.Role of ChIP-seq in the discovery of transcription factor binding sites, differential gene regulation mechanism, epigenetic marks and beyond. Cell Cycle. 13(18), 2847 – 52 (2014).
Park, P.J. ChIP-seq: advantages and challenges of a maturing technology. Nat. Rev. Genet. 10(10), 669 – 80 (2009).