Sequencing of micro RNA (small RNA)
Regulation of the gene expression
Your regulome project
Sequencing of non-coding RNAs
Strategy
- Illumina HiSeq 2000 for the analyses of small non-coding RNAs like miRNAs (micro RNAs), siRNAs (short interfering RNAs) or piRNAs (piwi-interacting RNAs)
- Roche GS FLX for the identification and analysis of known and unknown non-coding RNAs
Starting material
- Tissue
- Total RNA
- Small RNA
Bioinformatics service
- Supply of the raw data (file format fasta, fastq, sff (GS FLX) or fasta, fastq (Illumina))
- Clustering (representative of each cluster (fasta), clustering table (tsv))
- Identification of known miRNAs using Ssearch (number of hits to the same miRNA (tsv))
- BLAST homology searches (annotation table (tsv))
- Mapping against reference (mapping report (txt))
Spotlight
Customer Service
(D) +49 - 7531 81 60 68
(F) +33 - 4 91 82 84 88
(GB) +44 - 207 691 4921
(S) +46 - 8 655 3609
Opening hours:
8 am - 6 pm CET (Mon-Fri)
NEW! Sequencing Service featuring PacBio RS
Next Generation Sequencing Seminar
Next Gen Specialists on side
Genomics & Transcriptomes
Analyses & Visualisation
On the path to a deep understanding of the organism, two parameters are important to the interpretation of the Next Gen sequencing data: The use of various Next Gen systems to take advantage of each technology and the combination of different bioinformatic tools and their stepwise application.
Interested? Like to book us for a seminar? Contact us!
