Pre-Sequencing | services prior to sequencing

DNA preparation, PCR purification, primer design & synthesis, DNA isolation from tissue, cDNA libraries, genomic libraries ...

Reliable, well-tried & fast: Order these services in combination with our DNA sequencing services:

Primer Synthese

• Primer synthesis (for sequencing at GATC)
• Max. 25nt
• Primer storage in your personal primer archive at GATC for future sequencing orders 
• Delivery time two working days

Primer Design & Synthese

• Primer design & synthesis (for sequencing at GATC)
• Max. 25nt
• Primer storage in your personal primer archive at GATC for future sequencing orders 
• Delivery time two working days

PCRPurification

• Purification of plasmid DNA
• Within one working days

MiniPrep

• Purification of plasmid DNA
• Within two working days

PCR

• For metagenome analyses (16S and 18S RNA)

DNA and RNA isolation

• From tissue, cell cultures, cells, blood, PCR preparation
• Isolation of genomic DNA

BAC preparation / BAC Miniprep

• Isolation of BAC-DNA for sequencing  

cDNA  libraries

• Standard full length cDNA libraries for all sequencing technologies
• Normalized cDNA libraries
• Subtracted cDNA libraries (one and both directions)
• Prokaryotic cDNA
• 3’UTR, 5’UTR libraries (incl. SAGE and CAGE)
• Cloning tags
• cDNA library amplification and DNA preparation

Genomic libraries

1. Shotgun libraries of plasmids (800bp to 5kb)
2. Fosmids (to 40kbp)

Plating/Picking

• Plating, picking and replication in 96-well and 384-well plates
• Hitpicking per 96-well target plate

• Pre-Sequencing
• Single Reads
• Genomes
• Targeted (re)-sequencing
• Human Sample Sequencing
• Transcriptomes
• Metagenome
• Regulome

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