Sequencing of prokaryotic cDNA
Analysis of the metabolic activities of a metagenome
Your metagenome project
You wish to obtain an initial overview of the transcriptome of a metagenome (metabolic activity)
Strategy
Sequencing with Roche GS FLX
Sequencing
Using Roche GS FLX (Titanium protocol, single reads) with 15x to 20x coverage
Starting material
- Tissue
- Total RNA
- mRNA
- Prokaryotic cDNA
Bioinformatics service
- Supply of the raw data (file format fasta, qual, sff)
- De novo assembly (tools used depend on project, file format: ace)
- Clustering (table of the cluster distribution, diagrams (file format fasta, xls, txt, png))
- Table of the BLAST analysis (file format xls, txt)
- Bioinformatics statistics (contig and coverage distribution in pdf or xls file format)
Spotlight
Customer Service
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Next Generation Sequencing Seminar
Next Gen Specialists on side
Genomics & Transcriptomes
Analyses & Visualisation
On the path to a deep understanding of the organism, two parameters are important to the interpretation of the Next Gen sequencing data: The use of various Next Gen systems to take advantage of each technology and the combination of different bioinformatic tools and their stepwise application.
Interested? Like to book us for a seminar? Contact us!
