Sequencing of 16S or 18S rRNA
How complex is the metagenome?
Your metagenome project
You want to obtain an initial overview of the complexity of the metagenome.
Strategy
Sequencing with Roche GS FLX+
Sequencing
Using Roche GS FLX+ (Titanium protocol, single reads) with 15x to 20x coverage
Starting material
- Tissue
- Total RNA
- PCR products from 16/18 S rRNA primers
Bioinformatics service
- Supply of the raw data (file format fasta, qual, sff)
- Clustering (table of the cluster distribution, diagrams (file format fasta, xls, txt, png))
- Bioinformatics statistics (contig and coverage distribution in pdf or xls file format)
Spotlight
Customer Service
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(GB) +44 - 207 691 4921
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Opening hours:
8 am - 6 pm CET (Mon-Fri)
NEW! Sequencing Service featuring PacBio RS
Next Generation Sequencing Seminar
Next Gen Specialists on side
Genomics & Transcriptomes
Analyses & Visualisation
On the path to a deep understanding of the organism, two parameters are important to the interpretation of the Next Gen sequencing data: The use of various Next Gen systems to take advantage of each technology and the combination of different bioinformatic tools and their stepwise application.
Interested? Like to book us for a seminar? Contact us!
