Identification of flanking genome regions via LAM-PCR
Amplification and sequencing of unknown regions in a genome
The patented method of LAM-PCR (linear amplification-mediated PCR) allows the amplification and sequencing of unknown regions in every given genome, no matter if from a bacterium, plant, animal or human.
Your project
You would like
- to identify the sequence of an unknown genomic region flanking a known genomic segment
- to localise the integration site of a vector in a given genome
- to have a reliable method with great prospect of success, because you already tried other methods, like inverse PCR, but without success
Our service
- Linear amplification of the region flanking the known segment
- Purification of the amplification products
- Second strand synthesis
- Restriction digestion of the ds cDNA with an appropriate enzyme to reduce the fragments to a suitable size and for linker attachment for PCR
- Ligation of a linker cassette to attach a known primer binding site
- Specific amplification of the unknown region
- Sequencing of the PCR products
You receive
- the sequence of the 5’ and / or 3’ flanking region of my known genomic segment
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