Required DNA concentration and volume
Run24 | Run24Barcode | Run24Supreme
Concentration:
Plasmides DNA: 30-100ng/µl
PCR products: 10-50ng/µl
Customer primer: 10pmol/µl
Volume:
DNA samples & primers in separate tubes: 30µl each (sufficient for 8 reactions)
Please send the DNA dissolved in water. The solution must not contain any EDTA!
Templates/primers in 1.5ml reaction tubes.
BigRun
Concentration:
Cosmides/BAC/PAC: >100ng/µl
Lambda DNA: >150ng/µl
Customer primer: 10pmol/µl
Volume:
DNA samples & primers in separate tubes: 30µl each (sufficient for 8 reactions)
Please send the DNA dissolved in water. The solution must not contain any EDTA!
Templates/primers in 1.5ml reaction tubes.
GenomeRun
Concentration:
genomic bacterial/viral DNA (< 5Mb): >300ng/µl
Volume:
DNA samples & primers in separate tubes: 30µl each (sufficient for 8 reactions)
Please send the DNA dissolved in water. The solution must not contain any EDTA!
Templates/primers in 1.5ml reaction tubes.
SequenceYourself
At least eight non-cleaned sequencing reactions (BD Vers. 3.1). Templates to be send in 1.5ml reaction tubes
One Shot Primer Walking
Concentration:
Plasmid DNA: 100ng/µl
If you like to send your own primers, please contact us (sequencing hotline +49 7531 81 60 88).
Volumen:
40µl (insert size 1kb to 3,1kb)
70µl (insert size 3,1kb to 5,2kb)
90µl (insert size 5,2kb to 6,6kb)
Please send the DNA dissolved in water. The solution must not contain any EDTA!
Single strand sequencing
Concentration:
Plasmid DNA 100ng/µl
Cosmides/BAC/PAC > 100ng/µl
Lambda DNA > 150ng/µl
We do not accept customer primers.
Volume:
60µl (for an insert size of up to 4kb, if agar stab culture is not provided)
Please provide
- the reference sequence of the insert (if known)
- name & sequence of the cloning vector
- an agar stab culture of the clone if insert > 4kb
- templates in 1.5ml reaction tubes
- the filled out GVO document, only necessary if agar stab cultures are provided
Double strand sequencing
Concentration:
Plasmid DNA 100ng/µl
Cosmide/BAC/PAC > 100ng/µl
Lambda DNA > 150ng/µl
We do not accept customer primers.
Volume:
60µl (for an insert size of up to 2.5kb, if agar stab culture is not provided)
Please send
- the reference sequence of the insert (if known)
- name & sequence of the cloning vector
- an agar stab culture of the clone if insert > 2.5kb
- templates in 1.5ml reaction tubes
- the filled out GVO document - only necessary if agar stab cultures are provided
Run24Plate
Concentration:
Plasmid DNA: 30-100ng/µl
PCR products: 10-50ng/µl
Customer primer: 10pmol/µl
Volume:
DNA samples 30µl each (sufficient for 8 reactions). Please send the DNA dissolved in water. The solution must not contain any EDTA.
Primers 30ml (sufficient for 8 reactions, please contact our hotline for more information) in 1.5ml reaction tubes.
96 well and 48 well sequencing
Concentration:
Plasmid DNA: 30-100ng/µl
PCR products: 10-50ng/µl
Customer primer: 10pmol/µl
Volume:
DNA samples: 30µl
Primers: 50-100µl
Please send the DNA dissolved in water. The solution must not contain any EDTA!
Templates in 96 well microtitre plates. Please send agar stab cultures of the clones if GATC is to do the DNA preparation.
